The separation, from background, of response evoked by stimulation

R.M. Everson, B.W. Knight and L. Sirovich

Abstract

We consider the problem of estimating a small stimulus-induced response to stimulation which is masked by a fluctuating background when measurements of the background in the absence of stimulation are available, as is common in optical imaging of the cortex and in many other experimental situations. Two related methods based on the Karhunen-Loeve procedure are discussed. One seeks the function, an indicator function, that is most parallel to the response data and most orthogonal to the background data. The second removes the subspace spanned by the background from the response. Numerical investigations on simulated optical imaging data show that the first method is generally superior. Connections between the two methods and techniques for assessing the quality of the result are discussed.

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